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Korean Journal of Otorhinolaryngology-Head and Neck Surgery > Volume 37(6); 1994 > Article
Korean Journal of Otorhinolaryngology-Head and Neck Surgery 1994;37(6): 1135-41.
Antigens of Inner Ear Autoimmunity in the Cow's Inner Ear, Brain, Kidney and Liver
Sang Won Yeo, MD, Woo Jung Yoo, MD, Kwang Jae Cho, MD, Byung Woo Kim, MD, and Byung Do Suh, MD
Department of Otolaryngology, Catholic University Medical College, Seoul, Korea
암소의 내이, 뇌, 신장 및 간장에 존재하는 내이 자가면역의 항원
여상원 · 유우정 · 조광재 · 김병우 · 서병도
가톨릭대학교 의과대학 이비인후과학교실

In our pursuit to purify autoantigens and examine the presence of autoantigens in different organs, we investigated two extraction techniques to provide both water-soluble and SDS-soluble fractions of bovine inner ear, brain, kidney and liver. We have also examined the sera of patients with autoimmune sensorineural hearing loss for the presence of autoantibodies using Western blotting immunoassay against antigen extract of bovine inner ear, brain, kidney and liver. In immunoblots, all tissue extracts contain 68kD non-organ specific autoantigen. In addition to 68kD protein, inner ear includes 50kD and 33-35kD proteins, and brain includes 50kD, 37kD, 36kD and 35kD proteins, and kidney contains 50kD protein, and liver also contains 32kD protein. The results suggest that 68kD protein is a non-organ specific autoantigen. The SDS-soluble fractions are ideal for immunoassays in clinical study because the water-soluble fractions contain higher concentration of bovine serum albumin from blood contamination during tissue dessection. 

Keywords: AutoantigenWestern blotting immunoassayAutoimmune sensorineural hearing loss.
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Related article
Inner Ear Autoimmunity in Patients with Inner Ear Diseases  1995 ;38(2)
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