The Her-2/neu oncogene demonstrated a close relationship to the human epidermal growth factor receptor and it was first identified as a dominant transforming gene in chemically induced adrenal neuroblastomas of neonatal mice. It has been reported that patients with breast cancer showing such overexpression and amplification have a poorer prognosis than those without overexpression and amplification. There are few reliable prognostic makers of biological aggresiveness for head and neck tumors including salivary gland tumors. Eight cases of benign salivary gland tumors and 18 cases of malignant salivary gland tumors were analyzed for a molecular genetic alteration, Her-2/neu gene ampilfication by FISH(fluorescence in situ hybridization) and overexpression by immunohistochemitry results were compaired with clinically important rik factors. The results were as follows ; 1) Overexpression was observed in 3 of 8(38%) benign salivary gland tumors and in 13 of 18(72%) malignant salivary gland tumors. But this difference was not statistically significant(p>0.05). 2) Gene amplification was observed in 0 of 8(0%) benign salivary gland tumors and in 10 of 18(56%) malignant salivary gland tumors. This difference was statistically significant(p=0.007). 3) Gene amplification was observed in malignant salivary gland tumors were not correlated with age, gender, tumor size and L.N. metastasis(p>0.05), but overexpression was found more frequently in high grade malignant salivary gland tumors(p=0.011). In conclusion, overexpression and gene amplification of Her-2/neu in the salivary gland tumors are not enough for the prognosticatior.